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^ 2.3.2.2 Properties of the Estimator By construction, a is unbiased under ID1a and ID2, and if, in addition, we assume ID1b its variance is simply Var ^ a s2 w2 : j j 2:24

in steroid synthesis. Below are examples of how selected toxicants affect various susceptible targets of the male reproductive tract.

Note that the variance is a function of the amount of data, the nj , but is independent both of the data itself, Eq. (2.22), and of the actual (unknown) value of a. It is also independent of the precise choice of mother wavelet, except indirectly through the choice of N , the number of vanishing moments. A quantitative study of this dependence is given by Delbeke and Abry [27].

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occurrence in other RNA virus infections is largely unexplored, but probably is not related to the activity described below as RLI Bisbal et al78 83 isolated a polypeptide inhibitor (termed RLI) of the 2-5A system based on their screening of an expression library assayed by binding to radioactive 2-5ApCp This protein was proposed as a regulator that would inhibit the binding of 2-5A to RNase L, thereby blocking activation of RNase L and its nuclease activity Although RLI had a poor af nity for RNase L, it may associate directly but noncovalently with the enzyme to alter its activation potential by 2-5A Overexpression of RLI in HeLa cells partly antagonized the anti-picornaviral effects of interferon, whereas RLI antisense constructs partly blocked downregulation of the 2-5A/RNase L pathway in EMCV-infected cells RLI increased during human immunode ciency virus type 1 (HIV-1) infection.

Epidemiological data have indicated that wives of men exposed to the compound vinyl chloride experience spontaneous abortion. Vinyl chloride is used to manufacture polyvinyl chloride (PVC). PVC is a component of various plastic products, including pipes, furniture, and automobile upholstery. Ethylnitrosourea (ENU) is a mutagenic agent that has been used extensively in mouse mutagenesis. ENU acts on male spermatogonial stem cells, introducing mutations. It is also capable of inducing reversible sterility in mice. Actinomycin D is an older chemotherapeutic drug that has been used in cancer therapy for many years. Actinomycin D is commonly used in the treatment of gestational trophoblastic cancers, testicular cancer, Wilm s tumor, and rhabdomyosarcoma. Actinomycin D intercalates with DNA and disrupts the structure and function of the DNA of the spermatozoa.

It has been shown [75], in the limit of nj large for each j in j1 ; j2 , that the ^ Cramer Rao bound for the full problem is attained, showing that a is asymptotically the minimum variance unbiased estimator under ID1 ID2. The decrease in variance ^ of a as a function of the size of the data is then explicitly seen to be 1=n, a remarkable result, being the rate of decay typical of SRD problems, yet appearing in a dif cult scaling context. Numerical comparisons [75] show that away from the ^ limit the variance of a remains extremely close to the Cramer Rao bound. This is not surprising as the assumption that nj is large is a very good one, except possibly for the nj corresponding to the largest j, since nj 1 % nj =2. Examining the limit in more detail, for large nj we have [75] log2 e nj 2 log2 e 2 : nj

The antibiotics and antimetabolites used in cancer treatment (i.e., vinblastine) are spermatotoxic and affect semen quality. Ionizing radiation is also spermatotoxic (with spermatogonial cells being the most sensitive). Prolonged scrotal heating is a factor that affects the earlier states of spermatogenesis.

2:25 2:26

This might be related to the downregulation of RNase L activity that has been described previously Overexpression of RLI caused a decrease in RNase L activity and a twofold enhancement of HIV production The HIV replication increase correlated with enhancement of levels of HIV RNA and proteins To the contrary, reduction of RLI levels in RLI antisense cDNA-expressing clones reversed the inhibition of RNase L activity associated with HIV multiplication and led to a threefold diminution in the viral load and a decrease in HIV RNA and proteins ABCE homologues, subfamilies of ATP binding cassette (ABC) transporters, have been identi ed in 37 species that apparently lack an RNase L84 ABCE is the proposed inhibitor of RNase L identi ed by Bisbal and co-workers Thus, RNase L inhibition must not be the only functional role of ABCE.

Anabolic steroids, antidepressants and drugs of abuse affect libido, potency, and ejaculatory function. Anabolic steroids are derivatives of testosterone, and have strong genitotropic effects. There is published evidence indicating that anabolic steroids increases sexual desire; however, the frequency of erectile dysfunction is also increased. Treatment with the antidepressant uoxetine has been associated with sexual side effects including delayed or nonexistent ejaculation and hyposexuality. Mice treated in utero with the anitleukemic agent 5-aza-2 -deoxycytidine exhibit abnormal reproductive behavior and low reproductive capacity.

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